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The 2 protein bands from 40 kDa to 30 kDa were being absent just after 24 h incubation at 45 °C. For the remainder of the bands, several concentrations of all three proteases retained protein bands akin to their primary molar masses (Determine 6a,b). The existence of bands at lower molecular weight in fish roe just before hydrolysis was unforeseen. While in the report by Ghelichi et al. [eight], small peaks of a relatively very low molecular body weight vary (3–fourteen kDa) were noticed in defatted carp roe homogenate, perhaps on account of an autolytic procedure taking place in fish roe and/or maybe the defatting process bringing about the formation of minimal-molecular-fat peptides.
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